Bioengineering silkworms with butterfly cytotoxic modified silk glands can produce sericin protein cocoons

Tech 2023-06-01 15:29:28 Source: Network

||(PSGs)pierisin-1A (P1A)P1AP1APSGP1APierisin-175%P1AADP[32P]NADADPDNAHPLCpierisin-1P1ADNA ADP5%P < 0.001P1ANDNA ADPP1A269B

(PSGs)pierisin-1A (P1A)P1A

P1APSG

P1APierisin-175%P1AADP[32P]NADADPDNAHPLCpierisin-1P1ADNA ADP5%P < 0.001

P1ANDNA ADPP1A269B. moriBM-NSpodoptera frugiperdaSf21

P1A269P1ABM-N

P1A269w1-pndP1A269/P1A269FibHP1A269PSGP1A269PSGP1APSGC

PCRDNA16SDS/PAGEP1A2696PSGP1A269P1A269Sf21BM-N

P1A2694367RT-PCRP1A269mRNA36

w1-pndP1A269/P1A269PSGw1-pnd+/+PSGw1-pndP1A269/P1A26967

3PSGFibH4

P1A269436P1A269PSG

w1-pndP1A269/P1A269

w1-pndP1A269/P1A269w1-pnd+/+79%P < 0.00170%[FibHFibL]25%

PSGw1-pndP1A269/P1A269

5-20%SDS/PAGEFibLFibLFibHw1-pnd+/+w1-pndP1A269/P1A2691-4

RT-PCRw1-pndP1A269/P1A269PSGFibHFibLmRNA5677w1-pndP1A269/P1A269FibHFibL mRNAw1-pnd+/+FibHFibL mRNA3.1%0.9%P < 0.001

w1-pnd+/+A3w1-pndP1A269/P1A269PSGmRNAP1A269PSGFibHFibL

w1-pndP1A269/P1A269w1-pnd+/+1.51.3P < 0.001

GlyThrSerTyr

w1-pndP1A269/P1A269

Takasu-2

P1A269

KWP1A269/P1A269Kinshu x Showaw1-pndP1A269/P1A269EGFPw1-pnd+/+

KWP1A269/P1A269FibLFibHSDS/PAGEFibL

KWP1A269/P1A269w1-pndP1A269/P1A2691.7P < 0.001w1-pndP1A269/P1A269
shigen.nig.ac.jp/silkwormbase/top.jsp

pHSDS/PAGESDS/PAGE6M LiBrw1-pndP1A269/P1A269

10%1

96%42-34

ESEB5rhLIFLIF

LIFEB5ALPEB5blasticidinblasticidin

EB5ALPALP

LIFEB5rhLIFEB5ALPALPrhLIF1418P0.0053BP0.05LIFEB5

w1-pndP1A269/P1A269PSGP1ADNA ADPP1A269P1APierisin-1

P1A269BM-NP1A269BM-N

BM-NP1A269DNA

P1A269PSGFibHFibL mRNAPSGP1A269FibHFibL

A3 mRNAP1A269P1A269P1A269P1A269PSG

P1A269A3 mRNAP1A269PSGrRNAsP1A26918S rRNA

Pierisin-1Pierisin-1

P1AP1A N

P1ANP1A

P1AP1APSGDNA ADP

BM-NP1A269Sf21PSGDNA ADPP1A269P1A269P1A

w1-pndP1A269/P1A269Bcl-2XPSG

T7 RNAPSGFibLFibHFibHFibHFibL

NdPSGFibHFibL

P1A

[1] Mineralization and Biocompatibility of Antheraea pernyi (A. pernyi) Silk Sericin Film for Potential Bone Tissue Engineering. Feng Liu;;Ali Goodarzi;;Haifeng Wang;;Joanna Stasiak;;Jianbo Sun;;Yu Zhou;;Mingying Yang;;Namita Mandal;;Yajun Shuai;;Guanshan Zhou;;Sijia Min;;Liangjun Zhu.Bio-Medical Materials and Engineering,2014
[2] Preparation and Characterization of Silk Sericin/Glycerol/Graphene Oxide Nanocomposite Film. Haesung Yun;;Moo Kon Kim;;Hyo Won Kwak;;Jeong Yun Lee;;Min Hwa Kim;;Eui Hwa Kim;;Ki Hoon Lee.Fibers and Polymers,2013
[3] Preliminary Characterization of Genipin-Cross-Linked Silk Sericin/Poly(vinyl alcohol) Films as Two-Dimensional Wound Dressings for the Healing of Superficial Wounds. Tippawan Siritientong;;Juthamas Ratanavaraporn;;Teerapol Srichana;;Pornanong Aramwit;;Joshua R. Mauney.BioMed Research International,2013
[4] Potential of 2D crosslinked sericin membranes with improved biostability for skin tissue engineering. Sunita Nayak;;Sarmistha Talukdar;;Subhas C. Kundu.Cell and Tissue Research,2012
[5] Evaluation of sericin/collagen membranes as prospective wound dressing biomaterial. Omer Akturk;;Aysen Tezcaner;;Hasan Bilgili;;M. Salih Deveci;;M. Rusen Gecit;;Dilek Keskin.Journal of Bioscience and Bioengineering,2011

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